Increasing Transient and Subsequent Stable Transgene Expression in Chrysanthemum Following Optimization of Particle Bombardment and Agroinfection Parameters.
نویسندگان
چکیده
منابع مشابه
Transient and stable transfection of Leishmania by particle bombardment.
Transfection of trypanosomatids, particularly the transfection of their mitochondrion and different developmental stages, is still a limiting step in many experiments using recombinant DNA technology. Two types of trypanosomatid transfection protocols have been described so far. The standard protocol is based on electroporation first described by Bellofatto and Cross [1]. The second protocol is...
متن کاملStable Transformation of Saintpaulia ionantha by Particle Bombardment
Background: A highly efficient genetic transformation system is essential for a successful genetic manipulation of the African violet (Saintpaulia ionantha Wendl.). Objectives: Developing a particle bombardment-based genetic transformation system for the African violet. Materials and Methods: A local cultivar of the African violet from Guilan province was used for transformation experiments. Th...
متن کاملeconomic optimization and energy consumption in tray dryers
دراین پروژه به بررسی مدل سازی خشک کردن مواد غذایی با استفاده از هوای خشک در خشک کن آزمایشگاهی نوع سینی دار پرداخته شده است. برای آنالیز انتقال رطوبت در طی خشک شدن به طریق جابجایی، یک مدل لایه نازک برای انتقال رطوبت، مبتنی بر معادله نفوذ فیک در نظر گفته شده است که شامل انتقال همزمان جرم و انرژی بین فاز جامد و گاز می باشد. پروفایل دما و رطوبت برای سه نوع ماده غذایی شامل سیب زمینی، سیب و موز در طی...
15 صفحه اولTransient expression of DNA and RNA in parasitic helminths by using particle bombardment.
Parasitic helminths (worms belonging to several metazoan phyla) cause considerable morbidity and mortality in humans. They are an important veterinary problem, and they result in significant economic losses in animal grazing and agriculture. Experimental studies on parasitic helminths have been limited by a lack of parasite cell lines and methods for molecular genetic analyses. We evaluated par...
متن کاملstable transformation of saintpaulia ionantha by particle bombardment
background: a highly efficient genetic transformation system is essential for a successful genetic manipulation of the african violet (saintpaulia ionantha wendl.).objectives: developing a particle bombardment-based genetic transformation system for the african violet.materials and methods: a local cultivar of the african violet from guilan province was used for transformation experiments. the ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Plant Biotechnology
سال: 2002
ISSN: 1347-6114,1342-4580
DOI: 10.5511/plantbiotechnology.19.229